Journal: bioRxiv
Article Title: Hydrogel array patterning using 3D-printed microfluidic inserts to control cell-cell and cell-ECM interactions
doi: 10.64898/2025.12.21.695682
Figure Lengend Snippet: (A) Orthogonal concentration gradients are formed by filling adjacent reservoirs with 10kDa (magenta, top reservoir) or 70kDa (red, right reservoir) fluorescent dextran solution in 3D-printed inserts placed on a tissue culture treated plate. (B) Representative images of 10kDa Cy5 dextran or (C) 70kDa Texas red dextran gradients at indicated timepoints. Scalebar: 1000µm. (D) Quantification of the 10kDa or (E) 70kDa dextran intensity profiles in the center of three primary hydrogel regions shown with crossed in panel (A). Error bars are representative of standard error of the mean of at least four devices.
Article Snippet: Solutions of 125μg/mL10kDa Cy5 dextran (Thermo Fisher Scientific, USA) and of 100μg/mL 70kDa Texas red fluorescent dextran (Thermo Fisher Scientific, USA) were diluted in Dulbecco’s Modified Eagle Medium (DMEM) cell culture medium.
Techniques: Concentration Assay